- 1(current)
Q. No.The cutting of DNA at specific locations became possible with the discovery of?
1. Restriction enzymes
2. Probes
3. Selectable markets
4. ligases
The DNA molecule to which the gene of interest is integrated for cloning is called:
| 1. | Transformer | 2. | Vector |
| 3. | Template | 4. | Carrier |
The introduction of t-DNA into plants involves?
1. Infection of the plant by Agrobacterium tumefaciens
2. Altering the pH of the soil, heat-shocking the plants
3. Exposing the plants to Cold for a brief period
4. Allowing the plant roots to stand in water
Which vector can clone only a small fragment of DNA?
1. Bacterial artificial chromosome
2. Yeast artificial chromosome
3. Plasmid
4. Cosmid
1. Ori-original restriction enzyme
2. Rop-reduced osmotic pressure
3. Hind III, Eco RI-selectable markers
4. AmpR, tetiR-antibiotic resistance genes
Which one of the following is a case of wrong matching?
| 1. | Somatic hybridization- Fusion of two diverse cells |
| 2. | Vector DNA- Site for tRNA synthesis |
| 3. | Micropropagation- In vitro production of plants in large numbers |
| 4. | Callus - Unorganized mass of cells produced in tissue culture |
5’___GAATTC___3’
3’___CTTAAG__5’
1. Deletion mutation
2. Start codon at the 5' end
3. Palindromic sequence of base pairs
4. Replication completed
1. DNA ligase
2. endonucleases
3. DNA polymerase
4. exonucleases
- 1(current)
Q. No.
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